P-35: Andrological Anomalies of Chromium and Ameliorations by Morus Nigra and Syzygium Cumini in Mice

Background Chromium (Cr) supplements used as “magic lift” without authentications and Cr contaminated sewage water cultivate vegetables and crops around the vicinity of big cities. It is scientific responsibility to find out the toxic effects and preventive measures against such pollutants by local cheapest sources. Testicular histopathology of hexavalent chromium (Cr+6) was investigated in adult male albino mice (Mus musculus) as mammalian model and the ameliorative effects of Mulberry (Morus nigra) fruit extract (MFE) and Jambul (Syzygium cumini) were studied against Cr+6 exposure. MaterialsAndMethods Fifty mice were grouped (n=10) as: Control, Cr-free drinking water (15 days); Cr, 50 ppm in drinking water (10 days) followed by no treatment (5 days); Cr-M, Cr-J, Cr-MJ treated as Cr group but followed by MFE, JFE and co-treatment (0.25 mL/12 h) respectively for next 5days. Animal recovered at 16th day to collect blood and testes for biochemical, hematological, histological and micrometrical observations. Results The Cr+6 treated animals showed atrophy and necrosis of seminiferous tubules (ST) to create inter-tubular spaces of testes and increased the testosterone level about 8 folds. The cross sectional area (CSA) of the ST was increased (29975.0 ± 2373.1μ2) as compare to control (27184.48 ± 488.4μ2) with dislodged parrot beak headed (PBH) spermatozoa as compared to club headed (CH) spermatozoa. The accumulation of debris along with tail-less spermatozoa within ST may cause lyse of ST at vacuolated fragile margin. The micrometric studies indicated that spermatogonia and primary spermatocytes were significantly (P≤0.001) decreased. The irregular distorted sperm heads with alteration of sperm tail length and mid-piece thickness indicated the anomalies of gametes and the possible intimations of infertility, which can be recovered by fruit extracts. Conclusion The histological, micrometric, lipid profile findings, enzymatic evaluations and liver function assessments clearly revealed that Mulberry Fruit Extract (MFE) and Jambul Fruit Extract (JFE) have ameliorative aptitude against andro-hepatic anomalies of hexavalent chromium.